mRNA levels of genes encoding proteins involved in glycerol metabolism in the rsf1 (respiratory factor 1) mutant compared to the isogenic wild-type parent strain (BY4741). Stl1p is the main glycerol transporter; although glycerol can pass directly through the membrane, Stl1p vastly increases the rate of translocation. Stl1p is induced nearly 1000-fold in the presence of glycerol (Roberts and Hudson 2006). Gut1p (cytosolic glycerol kinase and Gut2p (mitochondrial glycerol-3-phosphate) are required for glycerol utilization. Dak2p (dihydroxyacetone kinase) is involved in detoxification of the toxic intermediate dihydroxyacetone. Rsf2p is a transcription factor which is required for efficient glycerol utilization. Loss of Rsf1p and Rsf2p causes dysregulation of a similar panel of genes.
Original artwork and all microarray experiments were performed by George Gordon Roberts III.
Original artwork and all microarray experiments were performed by George Gordon Roberts III.