The evolutionarily conserved protein family of AAA-proteins plays key roles in fundamental cellular pathways, including protein homeostasis, chromatin remodeling and vesicle trafficking. AAA-ATPases also fulfill crucial roles in ribosome biogenesis, a pathway that recently also entered the focus of chemical biology as a promising target (recently reviewed in ref. 8). The formation of the two ribosomal subunits is a long cascade of consecutive maturation steps with the aim to correctly process and assemble the ribosomal RNAs with all ribosomal proteins. Drg1 is a key factor in late eukaryotic ribosome biogenesis and binds to pre-60S particles, the precursors of the large ribosomal subunit, as soon as they are exported from the nucleus into the cytoplasm. Drg1 recognizes its substrate protein, the shuttling maturation factor Rlp24, on the pre-ribosomal particle via an unstructured, highly charged C-terminal extension of Rlp24. Here you can see a complete structure of Drg1 protein determined by single particle CryoEM (PDB code: 7NKU)
#molecularart ... #immolecular ... #ATPase ... #Drg1 ... #ribosome ... #biogenesis ... Rendered with @proteinimaging and finished with @corelphotophaint
#molecularart ... #immolecular ... #ATPase ... #Drg1 ... #ribosome ... #biogenesis ... Rendered with @proteinimaging and finished with @corelphotophaint
