Cas12i is a recently identified type V CRISPR-Cas endonuclease that predominantly cleaves the non-target strand of a double-stranded DNA substrate. This nicking activity of Cas12i could potentially be used for genome editing with high specificity. Cas12 is a compact and efficient enzyme that creates staggered cuts in dsDNA. Cas12 processes its own guide RNAs, leading to increased multiplexing ability. Cas12 has also been engineered as a platform for epigenome editing, and it was recently discovered that Cas12a can indiscriminately chop up single-stranded DNA once activated by a target DNA molecule matching its spacer sequence. This property makes Cas12a a powerful tool for detecting tiny amounts of target DNA in a mixture. Here you can see the recently resolved structure of Cas12i1 in complex with a synthetic RNA molecule from Escherichia coli (PDB code: 7D2L)
#molecularart ... #immolecular ... #CRISPR ... #Cas12i1 ... #catalyticcomplex ... #RNA... Rendered with @proteinimaging and finished with @corelphotopaint
#molecularart ... #immolecular ... #CRISPR ... #Cas12i1 ... #catalyticcomplex ... #RNA... Rendered with @proteinimaging and finished with @corelphotopaint
